黄岩谊博士
联系电话：+86****4058
  • 所在单位：北京大学
  • 所在城市：北京
  • 邮箱：yan****@pku.edu.cn
  • 通讯地址：北京市海淀区燕园街道颐和园路5号
详细 · 介绍
Expert introduction
个人履历

2022.3-2024，北京大学生物医学前沿创新中心(BIOPIC)，副主任

2020.3-现在，北京大学化学与分子工程学院，教授

2016.8-现在，北京未来基因诊断高精尖创新中心(ICG)，研究员

2015-现在，北大-清华生命科学联合中心，研究员

2010-现在，北京大学生物医学前沿创新中心(BIOPIC)，研究员

2013-2020，北京大学工学院，教授

2006-2013，北京大学工学院，特聘研究员

2005-2006，美国斯坦福大学生物工程系，博士后

2002-2005，美国加州理工学院应用物理系，博士后

2002，北京大学，博士

1997，北京大学，理学学士

主要研究方向

黄岩谊课题组致力于发展应用于面向生命科学和医学研究的新技术，课题组当前的研究兴趣集中在微量样品多组学技术、微流控技术及类器官培养技术三个方面。我们一直在发展高效、新型的高通量DNA测序技术及其应用方法，特别是测序化学与设备研发、单细胞测序技术应用与器件研发、以及少量细胞的表观基因组学测序方法研发。我们同时大力发展大规模集成微流控芯片技术，并将其应用在生命科学关键问题的研究中，包括细胞培养及微环境控制，以及在单细胞层次上观察生命过程的随机性。我们还大力发展新的生物成像技术及图像处理算法，用于研究细胞及活生物体的动态过程。我们利用相干拉曼散射显微技术，可以无需标记来研究活生物体和活细胞内的三维化学成像，同时利用这样的技术来促进药物释放及癌症诊断的研究。

获奖及荣誉

2015，国家自然基金委员会，杰出青年科学基金

2014,  英国皇家化学会会士

2013，北京大学绿叶生物医学奖

2012，国家自然科学基金委员会，优秀青年基金

2010，第十二届霍英东青年教师基金

2009，北京大学青年教师教学演示竞赛，一等奖

2008，国家教育部新世纪优秀人才

2004，全国优秀博士学位论文

2003，国家自然科学二等奖，第四完成人

2001，北京大学学生五四奖章

2000，中国大学生五四奖学金

代表性论文及论著
  1. Ruoyan Li,# Lin Di,# Jie Li,# Wenyi Fan,# Yachen Liu, Wenjia Guo, Weiling Liu, Lu Liu, Qiong Li, Liping Chen, Yamei Chen, Chuanwang Miao, Hongjin Liu, Yuqian Wang, Yuling Ma, Deshu Xu, Dongxin Lin*, Yanyi Huang*, Jianbin Wang*, Fan Bai*, Chen Wu*. A body map of somatic mutagenesis in morphologically normal human tissues. Nature 2021, 597 (7876), 398-403.
  2. Lin Di, Yusi Fu, Yue Sun, Jie Li, Lu Liu, Jiacheng Yao, Guanbo Wang, Yalei Wu, Kaiqin Lao, Raymond W Lee, Genhua Zheng, Jun Xu, Juntaek Oh, Dong Wang, X. Sunney Xie*, Yanyi Huang*, Jianbin Wang*. RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids. PNAS 2020, 117, 2886-2893.
  3. Xiannian Zhang, Tianqi Li, Feng Liu, Yaqi Chen, Jiacheng Yao, Zeyao Li, Yanyi Huang, Jianbin Wang. VVV1. Comparative analysis of droplet-based ultra-high-throughput single-cell RNA-seq systems. Molecular Cell. 2019 73(13) 130-142.e5
  4. Geng, S (Geng, Shuang); Huang, YY (Huang, Yanyi).  From Mouth Pipetting to Microfluidics: The Evolution of Technologies for Picking Healthy Single Cells.ADVANCED BIOSYSTEMS. 2018  2(9)UNSP 1800099.
  5. Zhang, Y (Zhang, Yi); Gao, JX (Gao, Jiaxin); Huang, YY (Huang, Yanyi); Wang, JB (Wang, Jianbin). Recent Developments in Single-Cell RNA-Seq of Microorganisms.  BIOPHYSICAL JOURNAL,  2018 115(2)173-180.
  6. Jiang, Dongqing; Zhang, Xiannian; Pang, Yuhong; Zhang, Jianyun; Wang, Jianbin; Huang, Yanyi. Terminal transfer amplification and sequencing for high-efficiency and low-bias copy number profiling of fragmented DNA samples. Protein & cell.  2018.
  7. Chen, H (Chen, He); Yao, JC (Yao, Jiacheng); Fu, YS (Fu, Yusi); Pang, YH (Pang, Yuhong); Wang, JB (Wang, Jianbin); Huang, YY (Huang, Yanyi). Tagmentation on Microbeads: Restore Long-Range DNA Sequence Information Using Next Generation Sequencing with Library Prepared by Surface-Immobilized Transposomes. ACS APPLIED MATERIALS & INTERFACES.  2018  10(14)11539-11545.
  8. Chen Z, Liao P, Zhang F, et al. Centrifugal micro-channel array droplet generation for highly parallel digital PCR. Lab Chip 2017;17(2):235-40. doi: 10.1039/c6lc01305h
  9. Li S, Chen T, Wang Y, et al. Conjugated Polymer with Intrinsic Alkyne Units for Synergistically Enhanced Raman Imaging in Living Cells. Angew Chem Int Ed Engl 2017;56(43):13455-58. doi: 10.1002/anie.201707042
  10. Liao P, Huang Y. Digital PCR: Endless Frontier of ‘Divide and Conquer’. Micromachines 2017;8(8):231.
  11. Chen X, Huang Y. Editorial overview: Molecular imaging for seeing chemistry in biology. Curr Opin Chem Biol 2017;39:iv-v. doi: 10.1016/j.cbpa.2017.06.016
  12. Chen Z, Zhou W, Qiao S, et al. Highly accurate fluorogenic DNA sequencing with information theory-based error correction. Nat Biotechnol 2017;35(12):1170-78. doi: 10.1038/nbt.3982
  13. Chen T, Huang Y. Label-Free Transient Absorption Microscopy for Red Blood Cell Flow Velocity Measurement in Vivo. Anal Chem 2017;89(19):10120-23. doi: 10.1021/acs.analchem.7b01952
  14. Zheng C, Zhang X, Li C, et al. Microfluidic Device for Studying Controllable Hydrodynamic Flow Induced Cellular Responses. Anal Chem 2017;89(6):3710-15. doi: 10.1021/acs.analchem.7b00013
  15. Yang L, Ma Z, Cao C, et al. MR-seq: measuring a single cell’s transcriptome repeatedly by RNA-seq. Science Bulletin 2017;62(6):391-98. doi: https://doi.org/10.1016/j.scib.2017.01.029
  16. Wu AR, Wang J, Streets AM, et al. Single-Cell Transcriptional Analysis. Annu Rev Anal Chem (Palo Alto Calif) 2017;10(1):439-62. doi: 10.1146/annurev-anchem-061516-045228
  17. Li C, Yu Z, Fu Y, et al. Single-Cell-Based Platform for Copy Number Variation Profiling through Digital Counting of Amplified Genomic DNA Fragments. ACS Applied Materials & Interfaces 2017;9(16):13958-64. doi: 10.1021/acsami.7b03146
  18. Yujiao Dang,# Liying Yan,# Boqiang Hu,# Xiaoying Fan, Yixin Ren, Rong Li, Ying Lian, Jie Yan, Qingqing Li, Yan Zhang, Min Li, Xiulian Ren, Jin Huang, Yuqi Wu, Ping Liu, Lu Wen, Chen Zhang, Yanyi Huang,* Fuchou Tang,* Jie Qiao* .Tracing the expression of circular RNAs in human pre-implantation embryos. Genome Biology 17 130 (2016) .doi: 10.1186/s13059-016-0991-3 (2016) .doi: 10.1021/acs.analchem.6b00862
  19. Yusi Fu, Chunmei Li, Sijia Lu, Wenxiong Zhou, Fuchou Tang, X. Sunney Xie,* Yanyi Huang*. Uniform and accurate single-cell sequencing based on emulsion whole-genome amplification. Proceedings of the National Academy of Sciences of the United States of America 112 (38), 11923-11928 (2015) .doi: 10.1073/pnas.1513988112
  20. Xiaoying Fan,# Xiannian Zhang,# Xinglong Wu, Hongshan Guo, Yuqiong Hu, Fuchou Tang,* Yanyi Huang*. Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos. Genome Biology 16 148 (2015) .doi: 10.1186/s13059-015-0706-1
  21. Jie Shen,# Dongqing Jiang,# Yusi Fu,# Xinglong Wu,# Hongshan Guo, Binxiao Feng, Yuhong Pang, Aaron M. Streets, Fuchou Tang,* Yanyi Huang*. H3K4me3 epigenomic landscape derived from ChIP-Seq of 1,000 mouse early embryonic cells. Cell Research 25 (1), 143-147 (2015) .doi: 10.1038/cr.2014.119
  22. Aaron M. Streets, Xiannian Zhang, Chen Cao, Yuhong Pang, Xinglong Wu, Liang Xiong, Lu Yang, Yusi Fu, Liang Zhao,* Fuchou Tang,* Yanyi Huang.* Microfluidic single-cell whole transcriptome sequencing. PNAS 2014, published online. doi: 10.1073/pnas.1402030111
  23. Senlian Hong, Tao Chen, Yuntao Zhu, Ang Li, Yanyi Huang,* Xing Chen.* Live-cell stimulated Raman scattering imaging of alkyne-tagged biomolecules. Angew. Chem. Int. Ed. 2014, published online. doi: 10.1002/anie.201400328
  24. Aaron M. Streets, Yanyi Huang.* Chip in a lab: microfluidics for next generation life science research. Biomicrofluidics 2013, 7(1), 011302.
  25. Chunhong Zheng, Zhilong Yu, Ying Zhou, Louis Tao, Yuhong Pang, Tao Chen, Xiannian Zhang, Haiwei Qiu, Hongwei Zhou, Zitian Chen, Yanyi Huang.* Live cell imaging analysis of the epigenetic regulation of the human endothelial cell migration at single cell resolution. Lab on a Chip 2012, 12(17), 3063-3072.
  26. Zhilong Yu, Tao Chen, Xiannian Zhang, Dan Fu, Xin Liao, Jie Shen, Xinxing Liu, Bo Zhang, X. Sunney Xie, Xiaodong Su, Junyuan Chen,* Yanyi Huang.* Label-free chemical imaging in vivo: three-dimensional non-invasive microscopic observation of amphioxus notochord through stimulated Raman scattering (SRS). Chemical Science 2012, 3(8), 2646-2654.
  27. Chunhong Zheng, Jingwen Wang, Yuhong Pang, Jianbin Wang, Wenbin Li, Zigang Ge,* Yanyi Huang.* High-throughput immunoassay through in-channel microfluidic patterning. Lab on a Chip 2012, 12(14), 2487-2490. (featured as inside front cover)
  28. Yongfan Men, Yusi Fu, Zitian Chen, Peter A. Sims, William J. Greenleaf, Yanyi Huang.* Digital polymerase chain reaction in an array of femtoliter PDMS microreactors. Analytical Chemistry 2012, 84(10), 4262-4266.
  29. Wentao Li, Tao Chen, Zitian Chen, Peng Fei, Zhilong Yu, Yuhong Pang, Yanyi Huang.* Squeeze-chip: A finger controlled microfluidic flow network device and its application to biochemical assays. Lab on a Chip 2012, 12(9), 1587-1590. (featured as inside front cover)
  30. Peng Fei, Zhilong Yu, Xu Wang, Peter J. Lu, Yusi Fu, Zi He, Jingwei Xiong, Yanyi Huang.* High dynamic range optical projection tomography (HDR-OPT). Optics Express 2012, 20(8), 8824-8836.
  31. Ying Zhou, Yuhong Pang, Yanyi Huang.* Openly Accessible Microfluidic Liquid Handlers for Automated High-throughput Nanoliter Cell Culture. Analytical Chemistry 2012, 84(5), 2576-2584.
  32. Chunhong Zheng, Liang Zhao, Gui’e Chen, Ying Zhou, Yuhong Pang, Yanyi Huang.* Quantitative Study of the Dynamic Tumor-endothelial Cell Interactions through an Integrated Microfluidic Coculture System. Analytical Chemistry 2012, 84(4), 2088-2093.
  33. Jie Shen, Ying Zhou, Tu Lu, Junya Peng, Zhixiang Lin, Lei Huang, Yuhong Pang, Li Yu, Yanyi Huang.* An Integrated Chip for Immunofluorescence and Its Application to Analyze Lysosomal Storage Disorders. Lab on a Chip 2012, 12, 317-324.
  34. Zuoyan Han, Wentao Li, Yanyi Huang, Bo Zheng. Measuring rapid enzymatic kinetics by electrochemical method in droplet-based microfluidic devices with pneumatic valves. Analytical Chemistry 2009, 81(14), 5840-5845.
  35. Jianbin Wang, Ying Zhou, Haiwei Qiu, Huang Huang, Changhong Sun, Jianzhong Xi, Yanyi Huang.* A Chip-to-chip nanoliter microfluidic dispenser. Lab on a Chip 2009, 9(13), 1831-1835.
  36. Yuanzi Wu, Yanyi Huang, Hongwei Ma. A facial method for permanent and functional surface modification of poly(dimethylsiloxane). Journal of the American Chemical Society 2007, 129(23), 7226-7227.
  37. Yanyi Huang, Piero Castrataro, Cheng-Chung Lee, Stephen R. Quake. Solvent resistant microfluidic DNA synthesizer. Lab on a Chip 2007, 7(1), 24-26.
  38. Wei Liang, Lan Yang, Joyce K. S. Poon, Yanyi Huang,* Amnon Yariv, Kerry Vahala. Transmission characteristics of a Fabry-Perot etalon-microtoroid resonator coupled system. Optics Letters 2006, 31(4), 510-512.
  39. Wei Liang, Yanyi Huang,* Yong Xu, Reginald K. Lee, Amnon Yariv. Highly-sensitive fiber Bragg grating refractive index sensors. Applied Physics Letters 2005, 86(15), 151122.
  40. Yanyi Huang, Yong Xu, Amnon Yariv. Fabrication of functional microstructured optical fibers through a selective filling technique. Applied Physics Letters 2004, 85(22), 5182-5184.
  41. Yanyi Huang,* George T. Paloczi, Joyce K. S. Poon, Amnon Yariv. Bottom-up softlithographic fabrication of three-dimensional multilayer polymer integrated optical microdevices. Applied Physics Letters 2004, 85(15), 3005-3007.
  42. Yanyi Huang,* George T. Paloczi, Amnon Yariv, Cheng Zhang, Larry R. Dalton. Fabrication and Replication of Polymer Integrated Optical Devices Using Electron-Beam Lithography and Soft Lithography. Journal of Physical Chemistry B 2004, 108(25), 8606-8613.
  43. Yanyi Huang,* George T. Paloczi, Joyce K. S. Poon, Amnon Yariv. Demonstration of flexible freestanding all-polymer integrated optical ring resonator devices. Advanced Materials 2004, 16(1), 44-48.
推荐专家